Bacterial Culture Medium:

Culture media is a solid/liquid substance used for cultivation, isolation, identification or storage of microorganisms.

The growth of bacteria in a culture media helps to identify pathogens and aid in the diagnosis of infection. Culture media are also used to determine susceptibility of antimicrobials.

 Basic Requirements of a Culture:

The successful growth of bacteria in a culture requires the presence of basic requirement such as nutrients, ions, moisture, correct PH, osmotic pressure and accessory growth factors. A culture media should mimic the natural environment required for bacterial growth.

 Types of Media

– Selective Media: Is a solid media that contains ingredients which inhibit growth of one organism while preventing the growth of another.

Selective medium is used to culture specimens from sites that have normal floral in order to prevent unwanted contamination from growing.

In cultivation the organisms must be provided with conditions that irritate those that face them when they invade the human body i.e if it is a pathogenic bacteria, should be kept in a temp similar to that of a human body, provided with food nutrients like fats, proteins, PH and others found in human tissues.

N.B These media is possible for bacterial and fungal cultivation.